sd malaria ag p.f rdt  (Meridian Bioscience)

Journal: Malaria Journal

Article Title: Persistence of Plasmodium falciparum HRP-2 antigenaemia after artemisinin combination therapy is not associated with gametocytes

doi: 10.1186/s12936-022-04387-0

Figure Lengend Snippet: Kaplan–Meier plots showing time to negativity by standard RDT, ultra-sensitive RDT and quantified HRP-2 between the ACT group (orange) and the ACT + PQ group (blue)

Article Snippet: RDT (SD/Alere, Yongin-si, Republic of Korea, Cat. 05EK50), the Alere Malaria Ag P.f RDT ULTRA SENSITIVE (SD/Alere, Yongin-si, Republic of Korea).

Techniques:

Journal: Malaria Journal

Article Title: Persistence of Plasmodium falciparum HRP-2 antigenaemia after artemisinin combination therapy is not associated with gametocytes

doi: 10.1186/s12936-022-04387-0

Figure Lengend Snippet: Test positivity and HRP-2 levels by study visit

Article Snippet: RDT (SD/Alere, Yongin-si, Republic of Korea, Cat. 05EK50), the Alere Malaria Ag P.f RDT ULTRA SENSITIVE (SD/Alere, Yongin-si, Republic of Korea).

Techniques: Concentration Assay

Journal: Nature Microbiology

Article Title: Plasmodium falciparum is evolving to escape malaria rapid diagnostic tests in Ethiopia

doi: 10.1038/s41564-021-00962-4

Figure Lengend Snippet: Characteristics of study participants and RDT results

Article Snippet: Among the 2,714 samples P. falciparum -positive by RDT, the northern region of Tigray had the highest proportion of infections with discordant RDT profiles at 140/689 (20.4%, 95% CI 17.5–23.7), followed by Amhara with 211/1342 (15.8%, 95% CI 13.9–17.8) and Gambella with 10/683 (1.5%, 95% CI 0.7–2.8), as shown in Fig. . Fig. 1 Distribution of P. falciparum- positive RDT results and discordant profiles suggestive of pfhrp2 / 3 gene deletions. a , Aggregated results from both RDTs, CareStart Pf / Pv (HRP2/ Pv -LDH) RDT and SD Bioline Malaria Ag P.f. (HRP2/ Pf -LDH) RDT, displayed by region for all P. falciparum infections ( n = 2,714).

Techniques:

Journal: Nature Microbiology

Article Title: Plasmodium falciparum is evolving to escape malaria rapid diagnostic tests in Ethiopia

doi: 10.1038/s41564-021-00962-4

Figure Lengend Snippet: a , Aggregated results from both RDTs, CareStart Pf / Pv (HRP2/ Pv -LDH) RDT and SD Bioline Malaria Ag P.f. (HRP2/ Pf -LDH) RDT, displayed by region for all P. falciparum infections ( n = 2,714). The ‘2 HRP2−, 1 Pf -LDH+’ discordant RDT profile indicates potential infection by pfhrp2 / 3- deleted P. falciparum . Triangles represent the enrolment sites, including 11 districts and the Kule refugee camp within the Itang district in Gambella. b , Percentage of study participants identified with P. falciparum infection by RDT who had the discordant RDT profile, by district.

Article Snippet: Among the 2,714 samples P. falciparum -positive by RDT, the northern region of Tigray had the highest proportion of infections with discordant RDT profiles at 140/689 (20.4%, 95% CI 17.5–23.7), followed by Amhara with 211/1342 (15.8%, 95% CI 13.9–17.8) and Gambella with 10/683 (1.5%, 95% CI 0.7–2.8), as shown in Fig. . Fig. 1 Distribution of P. falciparum- positive RDT results and discordant profiles suggestive of pfhrp2 / 3 gene deletions. a , Aggregated results from both RDTs, CareStart Pf / Pv (HRP2/ Pv -LDH) RDT and SD Bioline Malaria Ag P.f. (HRP2/ Pf -LDH) RDT, displayed by region for all P. falciparum infections ( n = 2,714).

Techniques: Infection

Journal: Nature Microbiology

Article Title: Plasmodium falciparum is evolving to escape malaria rapid diagnostic tests in Ethiopia

doi: 10.1038/s41564-021-00962-4

Figure Lengend Snippet: Assay results

Article Snippet: Among the 2,714 samples P. falciparum -positive by RDT, the northern region of Tigray had the highest proportion of infections with discordant RDT profiles at 140/689 (20.4%, 95% CI 17.5–23.7), followed by Amhara with 211/1342 (15.8%, 95% CI 13.9–17.8) and Gambella with 10/683 (1.5%, 95% CI 0.7–2.8), as shown in Fig. . Fig. 1 Distribution of P. falciparum- positive RDT results and discordant profiles suggestive of pfhrp2 / 3 gene deletions. a , Aggregated results from both RDTs, CareStart Pf / Pv (HRP2/ Pv -LDH) RDT and SD Bioline Malaria Ag P.f. (HRP2/ Pf -LDH) RDT, displayed by region for all P. falciparum infections ( n = 2,714).

Techniques: Polymerase Chain Reaction

Journal: Malaria Journal

Article Title: Diagnostic performance of an ultrasensitive HRP2-based malaria rapid diagnostic test kit used in surveys of afebrile people living in Southern Ghana

doi: 10.1186/s12936-021-03665-7

Figure Lengend Snippet: Prevalence of asymptomatic individuals. a Age categorized prevalence of P. falciparum in Obom and Simiw by the different diagnostic methods. b Prevalence of P. falciparum prevalence in pregnant women from Ewim and non-pregnant women of the same age group from Simiw and Obom (combined) by the different diagnostic tests. PCR: P. falciparum species PCR; micro: microscopy; UsmRDT: ultra-sensitive Alere™ Malaria Ag P.f RDT kit and mRDT, SD Bioline Malaria Ag P.f RDT kit

Article Snippet: PCR: P. falciparum species PCR; micro: microscopy; UsmRDT: ultra-sensitive Alere™ Malaria Ag P.f RDT kit and mRDT, SD Bioline Malaria Ag P.f RDT kit Categorizing the study participants from Obom and Simiw into younger children (12 years old and below), older children (13–17 years old) and adults (18 years old and above) showed a general inverse relationship of age group with parasite prevalence; with adults having the lowest prevalence of P. falciparum while children had the highest prevalence of the parasite (Fig. a).

Techniques: Diagnostic Assay, Microscopy

Journal: Malaria Journal

Article Title: Diagnostic performance of an ultrasensitive HRP2-based malaria rapid diagnostic test kit used in surveys of afebrile people living in Southern Ghana

doi: 10.1186/s12936-021-03665-7

Figure Lengend Snippet: Detection of P. falciparum by different diagnostic methods. All samples were grouped into sets belonging to PCR, UsmRDT (ultra-sensitive Alere™ Malaria Ag P.f RDT kit) and mRDT (SD Bioline Malaria Ag P.f RDT kit positive samples)

Article Snippet: PCR: P. falciparum species PCR; micro: microscopy; UsmRDT: ultra-sensitive Alere™ Malaria Ag P.f RDT kit and mRDT, SD Bioline Malaria Ag P.f RDT kit Categorizing the study participants from Obom and Simiw into younger children (12 years old and below), older children (13–17 years old) and adults (18 years old and above) showed a general inverse relationship of age group with parasite prevalence; with adults having the lowest prevalence of P. falciparum while children had the highest prevalence of the parasite (Fig. a).

Techniques: Diagnostic Assay

Journal: Malaria Journal

Article Title: Diagnostic performance of an ultrasensitive HRP2-based malaria rapid diagnostic test kit used in surveys of afebrile people living in Southern Ghana

doi: 10.1186/s12936-021-03665-7

Figure Lengend Snippet: Prevalence of asymptomatic individuals. a Age categorized prevalence of P. falciparum in Obom and Simiw by the different diagnostic methods. b Prevalence of P. falciparum prevalence in pregnant women from Ewim and non-pregnant women of the same age group from Simiw and Obom (combined) by the different diagnostic tests. PCR: P. falciparum species PCR; micro: microscopy; UsmRDT: ultra-sensitive Alere™ Malaria Ag P.f RDT kit and mRDT, SD Bioline Malaria Ag P.f RDT kit

Article Snippet: In this study, we utilized the ultra-sensitive Alere™ Malaria Ag P.f RDT (UsmRDT) kit in addition to the SD Bioline Malaria Ag P.f RDT (mRDT) kit, microscopy and PCR of the P. falciparum 18S rRNA gene to identify malaria parasite prevalence in afebrile study participants living in the south of Ghana.

Techniques: Diagnostic Assay, Microscopy

Journal: Malaria Journal

Article Title: Diagnostic performance of an ultrasensitive HRP2-based malaria rapid diagnostic test kit used in surveys of afebrile people living in Southern Ghana

doi: 10.1186/s12936-021-03665-7

Figure Lengend Snippet: Detection of P. falciparum by different diagnostic methods. All samples were grouped into sets belonging to PCR, UsmRDT (ultra-sensitive Alere™ Malaria Ag P.f RDT kit) and mRDT (SD Bioline Malaria Ag P.f RDT kit positive samples)

Article Snippet: In this study, we utilized the ultra-sensitive Alere™ Malaria Ag P.f RDT (UsmRDT) kit in addition to the SD Bioline Malaria Ag P.f RDT (mRDT) kit, microscopy and PCR of the P. falciparum 18S rRNA gene to identify malaria parasite prevalence in afebrile study participants living in the south of Ghana.

Techniques: Diagnostic Assay